Early alcoholic liver injury: formation of protein adducts with acetaldehyde and lipid peroxidation products, and expression of CYP2E1 and CYP3A

The formation of protein adducts with reactive aldehydes resulting from ethanol metabolism and lipid peroxidation has been suggested to play a role in the pathogenesis of alcoholic liver injury. To gain further insight on the contribution of such aldehydes in alcoholic liver disease, we have compared the appearance of acetaldehyde, malondialdehyde, and 4-hydroxynonenal adducts with the expression of cytochrome P-450IIE1, and cytochrome P-4503A enzymes in the liver of rats fed alcohol with a high-fat diet for 2 to 4 weeks according to the Tsukamoto-French procedure and in control rats (high-fat liquid diet or no treatment). Urine alcohol and serum aminotransferase levels were recorded, and the liver pathology was scored from 0 to 10 according to the presence of steatosis, inflammation, necrosis, and fibrosis. The ethanol treatment resulted in the accumulation of fat, mild necrosis and inflammation, and a mean liver pathology score of 3 (range: 1 to 5). Liver specimens from the ethanol-fed animals with early alcohol-induced liver injury were found to contain perivenular, hepatocellular acetaldehyde adducts. Malondialdehyde and 4-hydroxynonenal adducts were also present showing a more diffuse staining pattern with occasional sinusoidal reactions. In the control animals, a faint positive reaction for the hydroxynonenal adduct occurred in some of the animals fed the high fat diet, whereas no specific staining was observed in the livers from the animals receiving no treatment. Expression of both CYP2E1 and CYP3A correlated with the amount of protein adducts in the liver of alcohol-treated rats. Distinct CYP2E1-positive immunohistochemistry was seen in 3 of 7 of the ethanol-fed animals. In 5 of 7 of the ethanol-fed animals, the staining intensities for CYP3A markedly exceeded those obtained from the controls. The present findings indicate that acetaldehyde and lipid peroxidation-derived adducts are generated in the early phase of alcohol-induced liver disease. The formation of protein adducts appears to be accompanied by induction of both CYP2E1 and CYP3A.     

Fungal spore transport through a building structure

The study carried out laboratory measurements with a full-scale timber frame structure to determine penetration of inert particles with size distribution from 0.6 to 4 microm and spores of Penicillium and Cladosporium through the structure. Pressure difference over and air leakage through the structure were varied. Measurements at moderate pressure differences resulted in the penetration factors within the range of 0.05-0.2 for inert particles, and indicated also the penetration of fungal spores through the structure. The measurements showed that the penetration was highly dependent on pressure difference over the structure but not on holes in surface boards of the structure. The results show that surface contacts between the frames and mineral wool may have a significant effect on penetration. The penetration was approximately constant within particle size rage of 0.6-2.5 microm, but particles with diameter of 4.0 microm did not penetrate through the structure at all even at a higher-pressure difference of 20 Pa, except in the case of direct flow-path through the structure. Results have important consequences for practical design showing that penetration of fungal spores through the building envelope is difficult to prevent by sealing. The only effective way to prevent penetration seems to be balancing or pressurizing the building. In cold climates, moisture condensation risk should be taken into account if pressure is higher indoors than outdoors. Determined penetration factors were highly dependent on the pressure difference. Mechanical exhaust ventilation needs a special consideration as de-pressurizing the building may cause health risk if there is hazardous contamination in the building envelope exists.     

A review: fungal exposure assessment in indoor environments

While the fungal exposure assessment was based on the determination of fungal propagules for a long time, recent progress has led to the development of methodology for other fungal agents, e.g. the fungal cell wall components, metabolites, and allergens, that may be responsible for health effects caused by fungal exposure. This review includes a summary of the sampling techniques and analytical methods that are currently used or are in progress for the fungal exposure assessment. Prospects for the future trends are also discussed. In the future, the development will focus on sampling techniques that allow longer sampling times, a higher sampling efficiency for relevant particle sizes, and better possibilities for a wide range of analyses. In addition, new or modified methodology based on chemical, immunochemical, and molecular biological techniques to measure fungal agents related to health effects will improve the understanding of biological responses caused by fungal exposure.     

Retrieval of leaf chlorophyll content in field crops using narrow-band indices: effects of leaf area index and leaf mean tilt angle

Reliable estimation of leaf chlorophyll-a and -b content (chl-a + b) at canopy scales is essential for monitoring vegetation productivity, physiological stress, and nutrient availability. To achieve this, narrow-band vegetation indices (VIs) derived from imaging spectroscopy data are commonly used. However, VIs are affected by canopy structures other than chl-a + b, such as leaf area index (LAI) and leaf mean tilt angle (MTA). In this study, we evaluated the performance of 58 VIs reported in the literature to be chl-a + b-sensitive against a unique measured set of species-specific leaf angles for six crop species in southern Finland. We created a large simulated canopy reflectance database (100,000 canopy configurations) using the physically based PROSAIL (coupling of PROSPECT and SAIL (Scattering by Arbitrarily Inclined Leaves) radiative transfer models) model. The performance of model-simulated indices was compared against airborne AISA Eagle II imaging spectroradiometer data and field-measured chl-a + b, LAI, and MTA values. In general, LAI had a positive effect on the strength of the correlation between chl-a + b and VIs while MTA had a negative effect in both measured and simulated data. Three indices (REIP (red edge inflection point), TCARI (transformed chlorophyll absorption ratio index)/OSAVI (optimized soil-adjusted vegetation index), and CTR6 (Carter indices)) showed strong correlations with chl-a + b and similar performance in model-simulated and measured data set. However, only two (TCARI/OSAVI and CTR6) were independent from LAI and MTA. We consider these two indices robust proxies of crop leaf chl-a + b.     

Modelling and evaluation of the fretting fatigue cracking risk in smooth spherical contacts

A numerical model for the calculation of fretting fatigue crack initiation is presented and compared with experiments. The model is focused on smooth sphere-on-plane contact in partial and gross slip conditions. It is based on Hamilton’s explicit stress equations and the multi-axial Dang Van and Findley fatigue criteria enhanced with a statistical size factor concept. Promising correlation was found between the model and the experimental results with quenched and tempered steel 34CrNiMo6. The model assumptions, limitations and general application are also discussed.     

Factors Controlling the Migration of Tailings-Derived Arsenic: A Case Study at the Yara Siilinjärvi Site

The behaviour of arsenic (As) derived from tailings was investigated at the Yara Siilinjärvi apatite mine and industrial site in eastern Finland. The study assessed factors influencing the migration and fate of As and compared the anthropogenic As load to the natural geogenic background. Environmental risks related to As were assessed by examining the As concentrations in humus, glacial till, aquatic sediments, groundwater, and surface water. The occurrence and fractionation of As and the presence of secondary precipitates and geochemical transformations in the tailings and in the ambient soil and sediment were evaluated by selective extraction. The water-derived emissions were evaluated by field measurements, hydrogeochemical analysis, and modelling. Results indicate elevated environmental risks due to dust and seepage emissions from the tailings since the concentrations and mobility of As and other potentially harmful elements (PHEs) such as Co, Ni, and Zn were elevated relative to the geogenic background. These elements were mainly associated with Fe (oxy)hydroxides in the soil and their mobility was closely linked to Fe biogeochemistry. Additionally, although the concentrations of As and PHEs were high in the tailings pond and seepage water, they decreased in ambient groundwater and surface water, indicating Fe (oxy)hydroxide stability. This was supported by hydrogeochemical modelling, which indicated precipitation of Fe oxides and hydroxides. According to speciation modelling, As was present mainly as toxic trivalent arsenious acid (H₃AsO₃) in groundwater and as the less toxic pentavalent As acid (H₂AsO₄ ^? and HAsO₄ ^2?) in surface water.     

Sorting multisets stably in minimum space

We consider the problem of sorting a multiset of sizen containingm distinct elements, where theith distinct element appearsn _i times. Under the assumption that our model of computation allows only the operations of comparing elements and moving elements in the memory,(n logn – _i=1 ^m n _i logn _i +n) is known to be a lower bound for the computational complexity of the sorting problem. In this paper we present aminimum space algorithm that sortsstably a multiset in asymptoticallyOptimal worst-case time. A Quicksort type approach is used, where at each recursive step the median is chosen as the partitioning element. To obtain a stable minimum space implementation, we develop linear-time in-place algorithms for the following problems, which have interest of their own: Stable unpartitioning: Assume that ann-element arrayA is stably partitioned into two subarraysA ₀ andA ₁. The problem is to recoverA from its constitutentsA ₀ andA ₁. The information available is the partitioning element used and a bit array of sizen indicating whether an element ofA ₀ orA ₁ was originally in the corresponding position ofA. Stable selection: The task is to find thekth smallest element in a multiset ofn elements such that the relative order of identical elements is retained.A preliminary version of this paper was presented at the3rd Scandinavian Workshop on Algorithm Theory, Helsinki, July 1992.     

Black Ultra-Thin Crystalline Silicon Wafers Reach the 4n2 Absorption Limit–Application to IBC Solar Cells

Cutting costs by progressively decreasing substrate thickness is a common theme in the crystalline silicon photovoltaic  industry for the last decades, since drastically thinner wafers would significantly reduce the substrate-related costs. In addition to the technological challenges concerning wafering and handling of razor-thin flexible wafers, a major bottleneck is to maintain high absorption in those thin wafers. For the latter, advanced light-trapping techniques become of paramount importance. Here we demonstrate that by applying state-of-the-art black-Si nanotexture produced by DRIE on thin uncommitted wafers, the maximum theoretical absorption (Yablonovitch's 4n² absorption limit), that is, ideal light trapping, is reached with wafer thicknesses as low as 40, 20, and 10 µm when paired with a back reflector. Due to the achieved promising optical properties the results are implemented into an actual thin interdigitated back contacted solar cell. The proof-of-concept cell, encapsulated in glass, achieved a 16.4% efficiency with an J_SC = 35 mA cm⁻², representing a 43% improvement in output power with respect to the reference polished cell. These results demonstrate the vast potential of black silicon nanotexture in future extremely-thin silicon photovoltaics.     

Propylene–ethylene random copolymers: Comonomer effects on crystallinity and application properties

For understanding the correlation chain from the polymer structure to the final application properties of propylene–ethylene random copolymers, four commercial grades with different ethylene content (0–8 mol %) but identical molecular weight distributions were investigated. Structural investigations concerning the comonomer distribution, using two different techniques (temperature rising elution fractionation and stepwise isothermal segregation), showed an increase of inhomogeneity with the total comonomer content, which was reflected for temperature rising elution fractionation in a widening of the elution temperature range. Crystallinity and crystallization speed studies confirmed the reduced overall crystallinity and increase of γ‐modification content as reported previously, but they also showed the reason for these effects in the distinctive decrease of the spherulitic growth speed. Good correlations of all these parameters to the mechanical performance of the four materials were obtained. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 95: 1073–1081, 2005     

Good maintenance of high-impact activity-induced bone gain by voluntary, unsupervised exercises: An 8-month follow-up of a randomized controlled trial

The purpose of this study was to evaluate whether premenopausal women's voluntary unsupervised aerobic and step training could maintain the skeletal benefits obtained by an 18-month supervised high-impact training, and if so, to what extent. Thirty women of the original 39 study subjects (i. e., persons who completed the preceding 18-month randomized training intervention and who volunteered to continue the training on their own for a further 8 months) and 19 women of the 45 original control subjects (i.e., persons who volunteered to continue as controls) were included. The study group trained an average of twice per week and the training consisted of regular aerobic and step classes provided by local fitness centers. Areal bone mineral density (BMD, g/cm2) was measured from the lumbar spine, femoral neck, trochanter area of the femur, distal femur, patella, proximal tibia, calcaneus, and dominant distal radius at baseline and after 18 and 26 months. During the extended 8-month follow-up, the BMD of the study group increased more at the femoral neck (the intergroup change was +0.9% at 18 months and +2.8% at 26 months, p = 0.004 for the change between 18 and 26 months) and remained at the 18-month level at the distal femur, patella, proximal tibia, and calcaneus. In these sites, the statistically significant changes during the entire 26 months of training were 1.7-4.0% in the training group as compared with the changes of -0.9-1.5% in the control group. In the lumbar spine, BMD decreased from the 18-month level in both groups. In conclusion, the significant BMD increases that were obtained by supervised 18-month high-impact training were effectively maintained with subsequent unsupervised regular aerobic and step classes (twice per week). The finding emphasizes the effectiveness and feasibility of self-controlled aerobic and step exercises in the primary prevention of osteoporosis among healthy premenopausal women.